Background
" IgD is a member of the immunoglobulin family, an antibody isotype, expressed on plasma membranes of immature B cells. In a secreted form it is found in very small amounts in blood serum. IgM and IgD are the first antibody isotypes expressed during B cell ontogeny. After leaving the bone marrow to colonize secondary lymphoid organs, B cells acquire surface IgD of the same specificity as surface IgM through alternative splicing of a pre-messenger RNA comprising V(D)J and both heavy chain constant μ (Cμ) and Cδ exons. After encountering antigen in secondary lymphoid organs, mature B cells transcriptionally down-regulate surface IgD. Secreted IgD might enhance immune protection by regulating B cell homeostasis and activation. IgD-deficient mice have fewer B cells, delayed affinity maturation, and weaker production of IgG1 and IgE. IgD homologs are present in all vertebrate taxa, except for birds. IgD gene organization and structural data demonstrates that IgD has an ancient origin. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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