Antigen Preparation
A recombinant protein of human HLA-DR
Background
"HLA-DR (Human Leukocyte Antigen – DR isotype ) is αβ heterodimeric cell surface glycoprotein, MHC class II cell surface receptor. Both α and β chains are anchored in the membrane, where α (heavy) chain is 36 kD and a β (light) chain is 27 kD. Each subunit contains two extracellular domains, a membrane-spanning domain and a cytoplasmic tail. The primary function of HLA-DR is to present peptide antigens. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells that lead to the production of antibodies against this peptide antigen. They therefore have a critical role in the initiation of the immune response. The complex of HLA-DR and peptide constitutes a ligand for the T-cell receptor (TCR). HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. The reduced HLA-DR expression on monocytes is considered to correlate with infectious complications and the development of sepsis. Studies have shown that 3–20% of HLA-DR molecules constitutively reside in the lipid rafts of various antigen-presenting cells. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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