Antigen Preparation
A recombinant protein of human CD9
Background
"CD9 is a glycoprotein, member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. Depending on the cell type and associated molecules, CD9 has a wide variety of biological activities such as cell adhesion, motility, metastasis, growth, signal transduction, differentiation, and sperm–egg fusion. CD9 is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils Tetraspanins CD9 and CD81 and also CD63 (subgroup 2b), are co-regulators of HIV-1-induced virus–cell or cell–cell fusion. Some studies highlighted CD9 interactions with other membrane proteins necessary for fertilization such as pregnancy specific glycoprotein 17 (PSG17) or beta1 integrins"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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