Antigen Preparation
A recombinant protein of human CD7
Background
"CD7, a 40 kD type I transmembrane glycoprotein, is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells, NK cells, hematopoietic progenitors, on acute lymphocytic leukemia (ALL). Expression levels of this cell surface glycoprotein and the serine protease, elastase 2 (ELA2), in the leukemic cells of patients with chronic myeloid leukemia (CML) have been associated with clinical outcome. According to some authors, CD7 is a negative prognostic marker in myeloid malignancies. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. CD7 molecule has also been shown to have costimulatory activity and to induce tyrosine and lipid kinase activities. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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