Antigen Preparation
A recombinant protein of human CD66c
Background
"Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CD66c) is a member of the carcinoembryonic antigen (CEA) family.T his heavily glycosylated molecule consists of two constant Ig-like domains and one variable Ig-like domain and it is anchored to the membrane via its glycosylphosphatidylinositol (GPI). Within the hematopoietic system, CD66c expression is limited to granulocytes and its precursors,where it serves homotypic and heterotypic adhesion, Ca2+ mediated signaling and is markedly upregulated from intracellular stores after activation. The stable expression pattern of CD66c has clinical value in B-acute lymphoblastic leukemia not only in the recognition of abnormal leukemia cells at primary diagnosis but also in monitoring of minimal residual disease during the treatment. Members of this family are widely used as tumor markers in serum immunoassay determinations of carcinoma. CD66c is expressed on normal mucosal epithelia of colon and also seen in normal lung, granulocytes and cancers of epithelial cell origin. CD66c shows differential expression with membrane positivity in normal colorectal epithelial cells and cytoplasmic expression in tumour cells. CD66c can be specifically used for colon carcinoma and may be a novel marker in colon carcinoma stem cell isolation."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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