Antigen Preparation
A recombinant protein of human CD64
Background
"CD64 is a 72 kD single chain type I glycoprotein. CD64 is a membrane receptor for the Fc region of immunoglobulin G. It is functionally unique as it is the only FcγR able to bind monomeric IgG with high affinity. FcγRI is also structurally distinct, containing an extracellular Ig-interactive region of three Ig-like in contrast to the two domains of the low affinity receptors FcγRII and FcγRIII. CD64 is found on macrophages, monocytes, and dendritic cells. It was shown that in a large Crohn’s disease cohort neutrophil CD64 index and soluble CD64 were significantly elevated during active gastrointestinal inflammation. Erythema Nodosum Leprosum neutrophils express CD64, presumably contributing to the immunopathogenesis of the disease"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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