Antigen Preparation
A recombinant protein of human CD127
Background
"CD127 is a 60-90 kD type I transmembrane glycoprotein. It is also known as IL-7 receptor α chain or IL-7Rα. The function of IL7R requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. CD127 plays a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID)."
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
0.5-5 µg/106 cells
|