Antigen Preparation
A recombinant protein of human CD11a
Background
"CD11a is also called ITGAL integrin subunit alpha L. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3; ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50)) and also functions in lymphocyte costimulatory signaling. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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