Antigen Preparation
"A synthetic peptide SR PST SSR RRA ISE derived from human MDM2. It is an identical sequence among human, mice and rat."
Background
"MDM2 is proto-oncogene nuclear-localized E3 ubiquitin ligase. It promotes tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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