Antigen Preparation
A synthetic peptide of internal segment of human GATA1 protein.
Background
"GATA1 belongs to the GATA family of transcription factors. The proteins comprise a group of transcription factors that are
related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core
element GATA. There are six vertebrate GATA proteins, designated GATA-1 to GATA-6. GATA-1 is the founding member of
the GATA family and is required for erythroid and megakaryocytic cell development by regulating the switch of fetal
hemoglobin to adult hemoglobin. Mutations in GATA-1 have been linked to many human diseases, including acute
megakaryoblastic leukemia in Down syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome. "
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|