Antigen Preparation
Cell membrane fraction of mouse PBMC
Background
"CD3e protein together with CD3 -gamma, -delta, and -zeta and T-cell receptor alpha/beta forms a T-cell receptor-CD3 complex and plays a essential role in signal transduction. CD3–ε chain is central to CD3 core assembly and full complex formation. This transmembrane protein is expressed on T-cells, NK-T cells and thymocytes. The CD3–ε polypeptide plays a critical role in T-cell development. Studies show that independent mutations of the human CD3–ε gene result in a T cell receptor/CD3 complex immunodeficiency. Research demonstrates that susceptibility to type I diabetes in women is associated with the CD3 epsilon locus on chromosome 11. Studies by several groups confirm that the cytoplasmic domains of the CD3ε and ζ chains are not suspended in the cytosol, but bound to the inner leaflet of the plasma membrane."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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