Antigen Preparation
A recombinant protein of human CD3
Background
"CD3 is a cluster of differentiation glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. This protein is present on virtually all peripheral blood T cells. CD3 antibody is commonly used as a phenotypic marker for human T cells. CD3 is a part of TCR complex, and is required for proper assembly, trafficking and surface expression of TCR complex. It is involved in T cell activation, proliferation and apoptosis pathway. CD3 is engaged in activating both the cytotoxic Tcells (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). CD3 complex mediates signal transduction."
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
0.5-5 µg/106 cells
|