Antigen Preparation
A recombinant protein of c-terminus human Cytokeratin-5
Background
"Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 5(K5) is expressed in mitotically active basal layer cells, along with its partner keratin 14 (K14), and their expression is down-regulated as cells differentiate. Keratin 5, which belongs to the type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains conexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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