PAX6

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Antigen Preparation
A synthetic peptide derived from human PAX6 protein.
Background
"PAX6 is a member of the homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause aniridia as well as Peter's anomaly, both ocular diseases."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
This antibody recognizes human PAX6 protein. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 604-250
Lot #:
Size: 100ug/200ul
Host: Mouse
Clone: E5B
Isotyping: IgG1
Applications: IHC(P)
Reactivity: Hu
Price: $ 245.00
REFERENCES
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