Antigen Preparation
A recombinant protein of CD 40 protein
Background
"CD40, a 48 kD type I glycoprotein, also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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