Antigen Preparation
A recombinant protein of CD33 protein
Background
"CD33, a 67 kD type I transmembrane glycoprotein, is a smallest member of a sialoadhesion immunoglobulin superfamily. It contains two Igâ€like extracellular domains and two ITIMâ€like sequences in their cytoplasmic domain. This receptor is expressed on cells of myeloid lineage, but it can also be found on some lymphoid cells. Wide subsets of mitogen†or alloantigenâ€activated human T and natural killer (NK) cells express CD33. CD33 is highly expressed on myeloidâ€committed cells of the bone marrow and circulating monocytes. CD33 expression is downâ€regulated to low levels on peripheral granulocytes and resident macrophages, and it is constitutive on DC. This expression pattern suggests a role of CD33 on myeloid differentiation and cellular function of monocyte and DC. Myeloid and lymphoid CD33 cDNA are identical.CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity. After phosphorylation. CD33 is capable of recruiting the protein tyrosine phosphatases Src homologyâ€2â€containing tyrosine phosphataseâ€1 (SHPâ€1) and SHPâ€2 and may function as an inhibitory receptor by coligation with CD64 on myeloid cells"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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