Antigen Preparation
A recobinant protein of human CD103.
Background
"Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation.CD 103 is expressd on Tcells. Dendritic cell (DC) expression of CD103 is thought to enable DC interactions with E-cadherin-expressing gastrointestinal epithelia for improved mucosal immunosurveillance. In studies CD103+ acted to guide CSC exosomes to target cancer cells and organs, conferring the higher metastatic capacity of clear cell renal cell carcinoma (CCRCC) to lungs, suggesting CD103+ exosomes as a potential metastatic diagnostic biomarker. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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