Antigen Preparation
A synthetic peptide from internal sequence of human CD309/VEGFR2 protein. This sequence is identical to mouse and rat.
Background
"Vascular endothelial growth factor receptor-2 (VEGFR-2), also known as CD309 antigen, the earliest known marker for vascular endothelia cells, is essential in vasculogenesis and angiogenesis. Binding of the dimeric VEGF-A to the extracellular domains of two monomeric receptors induces dimerization and activation of tyrosine kinase at tyrosine residues Y951, Y1054 and Y1059 in the kinase domain serve as positive regulatory sites. Phosphorylation at Y951 of the kinase insert is related to the migration of endothelial cells for tumor vascularization and growth. Cd309 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes"
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|