Uroplakin-3

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Antigen Preparation
A synthetic peptide from C-term of human uroplakin-3 protein
Background
"Uroplakin-3 is a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Urothelium is built with dimensional, asymmetric plaques, whose specific structure is crucial for tightness and elasticity of urothelium. Integral components of these plaques are uroplakins (UP). Several isoforms of UP are known in humans: UP1a, UP1b, UP2, UP3a, UP3b, and UP3c. Biased expression of UP3 was shown in prostate, urinary bladder and other tissues. Mutations in this gene may be associated with renal adysplasia. The urinary UPK3A levels are uniformly elevated in bladder cancer patients than in those of normal volunteers and patients with benign urological disorders. High expression level of preoperative serum Uroplakin III is associated with biologically aggressive bladder cancer."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
This antibody recognizes human Uroplakin-3 protein. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 603-950
Lot #:
Size: 100ug/200ul
Host: Mouse
Clone: ABM129
Isotyping: IgG1
Applications: IHC(P), WB
Reactivity: Hu
Price: $ 245.00
REFERENCES
Hideyasu Tsumura et al. Asian Pac J Cancer Prev 2015;16(4):1539-43
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