Multi-Drug Resistance Marker (P-Glycoprotein)
P-Glycoprotein
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Antigen Preparation
A recombinant protein containing four tandem repeats of the amino acid sequences close to C-terminus .
Background
"Multi-Drug Resistance Marker (P-Glycoprotein) is a 170 kD cell membrane protein of the multi-drug resistance gene, MDR-1. Studies have linked the presence of P-Glycoprotein with resistance to a wide variety of chemotherapeutic agents. P-Glycoprotein is also found in various concentrations in most normal tissues, suggesting that the primary role for this protein is in normal secretion of physiological metabolites. This mouse monoclonal antibody reacts with a 170 kDa p-Glycoprotein. P-glycoprotein exists as a soluble and membrane associated form. Soluble p-glycoprotein is detected in extracellular fluids of cancer patients, such as malignant ascites and serum."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
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SPECIFICITY
This antibody recognizes human Multi-Drug Resistance Marker (P-Glycoprotein) protein. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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