Antigen Preparation
Human peripheral lymocytes were used as for an immunogen.
Background
"The leukocyte common antigen (LCA/CD45) consists of a family of heavily glycosylated glycoproteins of apparent MW 180- 240kD). It is a transmembrane protein-tyrosine-phosphatase. The LCA family includes a group of proteins present on all mature B and T lymphocytes, thymocytes, macrophages, spleen, lymph node, chronic lymphatic leukemia cells, bone marrow, thymus and granulocytes. It is absent in brain, kidney, liver, heart, erythrocytes, platelets and normal serum. The leukocyte common antigen is an abundant lymphocyte surface antigen that has been reported to be involved in signaling through the T cell antigen receptor. CD45 may function in the regulation of L-selectin (CD62L), in regulation of B-lymphocyte negative and positive selection and in T-cell activation. It stains lymphocytes, monocytes, eosinophils and also neoplastic cells of lymphoid origin. Neoplastic B cells and T cells in leukemia and in non-Hodgkin's lymphomas stain positive. This antibody may be useful in the evaluation of malignant lymphoma and nonlymphoid tumors. Neoplastic B and T cells in leukemia and in non-Hodgkin's lymphoma stain positive and hence can be distinguished from sarcomas and carcinomas."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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