Antigen Preparation
a synthetic peptide corresponding to amino acids 416-433. It recognizes the sequence LGDEARFAGHNFRNPSVL
Background
"Enolase is a cytoplasmic enzyme with three immunologically distinct subunits designated as alpha, beta, and gamma. There are five isoenzymes of enolase in normal human tissues. The gamma-subunit enolase, known as neuron-specific enolase (NSE), is expressed primarily in neurons, in normal and n neoplastic neuroendocrine cells. In normal tissues, most neurons and their axonal and dendritic processes stain strongly positive for NSE,with the exception of Purkinje cells. Schwann cells, cells of the adrenal medulla and paraganglia also contain NSE. Endocrine cells of the skin (Merkel’s cells), respiratory and GI tract epithelium, pituitary parathyroid, pancreatic islets, and C cells of thyroid all stain positive for NSE. NSE has been demonstrated in ganglioneuromas, neuroblastomas, schwannomas and malignant melanomas. It is also present in pheochromocytomas and paragangliomas. Carcinoids, medullary thyroid carcinomas, pituitary adenomas, and endocrine tumors of the pancreas and GI tract all show positive immunoreactivity for NSE. NSE is found in neuroendocrine carcinoma of the skin (Merkel’s cell tumor) and small cell carcinoma of the lung. A number of non-neuronal and non-endocrine tumors may also express NSE, including meningiomas, astrocytomas, renal cell carcinomas, carcinoma and fibroadenoma of the breast, carcinoma of the ovary, and malignant lymphomas. NSE positivity should not be relied upon entirely as evidence of the neuronal or neuroendocrine differentiation of a given neoplasm."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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