Antigen Preparation
A synthetic peptide sequence comprising amino acids 301-321 of human androgen receptor.
Background
"The androgen receptor belongs to the nuclear receptor subfamily 3 group C member 4 which is encoded by the Ar (also known as Andr, Nr3c4, Tfm) gene. Androgen receptor (AR) is a hormone-activated transcription regulator that plays important roles in normal prostate development and function, as well as in malignant prostate transformation. The binding of an androgenic hormone (testosterone or dihydrotestosterone) induces a conformational change in AR, resulting its dissociation from heat shock proteins and subsequent translocation from the cytosol to nucleus, where it dimerizes and mediates either transcription activation or repression in a target gene-specific manner. The androgen insensitivity syndrome (AIS) or testicular feminization (TFM) is caused by a mutation of the androgen receptor gene located on the X chromosome"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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