Antigen Preparation
A recombinant protein of AMACR
Background
"Alpha-methylacyl-CoA racemase (AMACR) is an enzyme that interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. AMACR regulates metabolism of lipids and drugs. Studies showed that dietary branchedâ€chain fatty acids increased production of AMACR in prostate cancer cells, with catalytic activity also being increased. AMACR protein is detectable in semen ejaculate. The higher AMACR levels detected in cancer patients suggests that semen AMACR protein may be useful as a noninvasive test for prostate cancer. It was demonstrated that high expression of AMACR is a key adverse prognostic factor and also a potential therapeutic target in oral squamous cell carcinoma."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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