Antigen Preparation
A synthetic peptide conrresponding to internal segment of human Catenin-gamma
Background
"Plakoglobin, also known as junction plakoglobin, JUP, or gamma-catenin, is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. Human plakoglobin is 81.7 kDa in molecular weight and 745 amino acids long. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. In the literature, the role of gamma-catenin in cancer is controversial, both oncogenic and tumor suppressor functions have been described for γ-catenin. Recently was shown that catenin-gamma (JUP) deregulation has been correlated with the initiation and progression of various malignancies. It promotes the proliferation, migration, and invasion of oral squamous cell carcinoma (OSCC) cells and inhibits apoptosis. Moreover, high expression of the protein could be used as a potential prognostic marker of OSCC. Other studies show tumor-suppressive effect of γ-catenin in colorectal cancer both in vitro and in vivo."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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