Antigen Preparation
A recombinant protein of human tyrosinase
Background
"Human tyrosinase is a Type 1 membrane bound glycoenzyme that catalyzes the initial and rate-limiting steps of melanin production in the melanosome. The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. Uncontrolled tyrosinase activity during the synthesis of melanin results in increased melanin synthesis. Tyrosinase is an enzyme present in plant and animal tissues that catalyzes the production not only melanin but also other pigments from tyrosine by oxidation. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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