Antigen Preparation
A synthetic peptide conrresponding to internal segment of human TSC1
Background
"This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. Tuberous sclerosis 1 (TSC1) protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. Studies indicate that Tsc1 confers neuroprotection against ischemia by inducing autophagy."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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