Telomerase Reverse Transcriptase (TERT)
Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2,
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Antigen Preparation
A recombinant protein of human TERT
Background
"Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. TERT is the catalytic component of the holoenzyme complex that is involved in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. The telomerase reverse transcriptase is upregulated in the majority of human cancers and contributes directly to cell transformation. For example studies show that mutations in the human telomerase reverse transcriptase (hTERT) gene promoter have been reported in hepatocellular carcinoma."
PURIFICATION
The Mouse IgG is purified by Affinity Purification
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SPECIFICITY
This antibody recognizes human Telomerase Reverse Transcriptase (TERT) protein. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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