Antigen Preparation
A recombinant protein Sgt A
Background
"This gene encodes a highly conserved nuclear protein involved in kinetochore function and required for the G1/S and G2/M transitions. Human Sgt1 (Suppressor of the G2 allele of Skp1) has been found to have two isoforms: Sgt-1A and Sgt-1B. This protein interacts with heat shock protein 90. Sgt1 protein translocates to the nucleus due to heat shock and the Ca(2+)-bound form of S100A6 is required for Sgt1 translocation. S100A6-Ca(2+)-mediated Sgt1 dephosphorylation promotes its nuclear translocation, most likely due to disruption of the Sgt1-Hsp90 complex. SUGT1(Sgt-1) protein expression was shown in lung , testis, liver, and brain. It is overexpressed in human colorectal cancer."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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