Antigen Preparation
A recombinant protein PCNA
Background
"Proliferating cell nuclear antigen (PCNA) is found in the nucleus and is a cofactor of DNA polymerase delta. The PC10 monoclonal antibody reacts with PCNA. The protein has a central role in promoting faithful DNA replication, providing a molecular platform that facilitates the myriad protein-protein and protein-DNA interactions that occur at the replication fork. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA belongs to the family of DNA sliding clamps (β clamps), which are structurally and functionally conserved. PCNA is loaded around DNA by the conserved chaperone-like complex RFC. PCNA is a marker of cells with proliferative potential and is used for detection of proliferation status of tumor tissue."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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