Antigen Preparation
A recombinant protein NKX3.1
Background
"This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Nkx3.1 is a member of the NK2 class of homeodomain proteins and is expressed in development, being an early marker of the sclerotome and prostate gland. It has been shown to be a critical factor for prostate differentiation and function. NKX3.1 is predominantly localized to prostate epithelium. NKX3-1 contains two exons encoding a 234 amino acid 35-38 kDa protein including a homeodomain. NKX3.1 is a prostatic tumor suppressor gene located on chromosome 8p.Most studies have shown that staining for NKX3.1 protein is positive in the majority of primary prostatic adenocarcinomas.. One study showed that NKX3.1 staining with this antibody was highly sensitive and specific for high-grade prostatic adenocarcinoma when compared with high-grade urothelial carcinoma. This antibody was shown to be a highly specific and sensitive tissue marker of metastatic prostatic adenocarcinoma. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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