Antigen Preparation
A recombinant protein Napsin A
Background
"Napsin A is a member of the peptidase A1 family of aspartic proteases. The preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. Napsin A is expressed in lung and kidney. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma. In the recent years, napsin A has emerged as a useful and broadly utilized diagnostic marker, which is helpful for confirming lung origin in carcinomas of unknown primary and for distinguishing lung adenocarcinomas from other pulmonary neoplasms, such as squamous cell carcinoma and mesothelioma. Expression of napsin was found to be associated with a high degree of differentiation in adenocarcinoma. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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