Antigen Preparation
A synthetid peptide of MMP1
Background
"Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase is an enzyme, a member of the peptidase M10 family of matrix metalloproteinases. Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Collagenases are the only proteinases that specifically cleave the collagen triple helix, and are important in a large number of physiological and pathological processes. MMP-1 has an archetypal structure consisting of a pre-domain, a pro-domain, a catalytic domain, a linker region and a hemopexin- -like domain. The MMP1 encoded preproprotein is proteolytically processed to generate the mature protease. MMP1 can degrade interstitial collagen types I, II, and III, clearing a path for cancer cells to invade matrix barriers and migrate through tissue stroma. Studies show that MMP1-1607 (1G>2G) polymorphism is significantly associated with elevated risk of cancers. Mutations in this gene are also associated with chronic obstructive pulmonary disease (COPD). "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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