MAdCAM1
MACAM1,MAdCAM-1, hMAdCAM-1, mucosal addressin cell adhesion molecule 1, mucosal addressin cell adhesion molecule-1
Download Product PDF
MSDS Download Product PDF
Antigen Preparation
A recombinant protein MadCAM1
Background
"The MAdCAM1 mucosal vascular cell adhesion molecule 1 also known as addressin is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. MADCAM1 is selectively expressed on mucosal endothelial cells, driving memory T-cell re-circulation through mucosal tissues. It was shown recently that MAdCAM1 was also selectively expressed on α-smooth muscle actin-positive reticular framework between white pulp and the marginal zone in the human spleen. The reticular framework may function in lymphocyte homing and segregation into the periarteriolar lymphoid sheath, lymph follicle and marginal zone. Other studies confirm that MAdCAM-1 is up-regulated in cirrhosis with expression on endothelium of the peribiliary vascular plexus and lymphoid aggregates."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
|
SPECIFICITY
This antibody recognizes human MAdCAM1 protein. The other species are not tested.
|
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
|
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
|
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|