SOX-3
SRY (sex determining region Y)-box 3
Download Product PDF
MSDS Download Product PDF
Antigen Preparation
A synthetic peptide derived from N-terminus of Human SOX-3 protein.
Background
"The SOX3, a protein transcription factor, belongs to a member of the SRY-related HMG-box (SOX) family . SOX3 is 447 aa protein which has diverse roles in development. The SOX3 gene is involved in a large family in which affected individuals have mental retardation and growth hormone deficiency. The mutation results in an in-frame duplication of 33 bp encoding for 11 alanines in a polyalanine tract of the SOX3 gene. The expression pattern during neural and pituitary development suggests that dysfunction of the SOX3 protein caused by the polyalanine expansion might disturb transcription pathways and the regulation of genes involved in cellular processes and functions required for cognitive and pituitary development. SOX3 is expressed in the central nervous system from the earliest stages of development. It is one of the earliest neural markers in vertebrates and plays a role in specifying neuronal fate."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
|
SPECIFICITY
This antibody recognizes ~46 kDa of human SOX-3 protein. The other species are not tested.
|
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
|
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
|
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|