Antigen Preparation
"A synthetic peptide derived from internal sequence (90-160aa) of human TGFbeta receptor type-2. This sequence is also identical within human, rat, mouse, chicken, bovine species."
Background
"Transforming growth factor β isoforms (TGF-β1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal through a highly restricted subset of receptors known as TGF-β type I receptor (TβR-I) and TGF-β type II receptor (TβR-II). TGF-β's specificity for TβR-I has been proposed to arise from its pre-helix extension, a five-residue loop that binds in the cleft between TGF-β and TβR-II. TGF-β binds with high affinity to the type II receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, which requires both its kinase activity and association with another TGF betabinding protein, the type I receptor, to signal growth inhibition and early gene responses. Receptors I and II associate as interdependent components of a heteromeric complex: receptor I requires receptor II to bind TGF beta, and receptor II requires receptor I to signal. Mutations in TβR-I and TβR-II gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS)."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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