Pdia 4
protein disulfide isomerase family A, member 4
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Antigen Preparation
"A synthetic peptide corresponding to the C-terminus of PDIA4 protein. This sequence is identical to human, rat, mouse, bovine, dog, and zebra fish."
Background
"Protein Disulfide Isomerase A4 (PDIA4 or ERp72), a 645aa protein, is an endoplasmic reticulum luminal protein that responds to stress. Sequence analysis shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. . "
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody recognizes ~75 kDa of PDIA4. It reacts with human, rat and mouse. The other species are not tested. "
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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