GPCR119
G-Protein coupled receptor 119; Gpr119;
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Antigen Preparation
A synthetic peptide corresponding to the extracellular domain of mouse GPR 119 protein. This sequence is identical to rat.
Background
"G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intra cellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane- spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR119 is a 335 amino acid protein that is mainly expressed in the pancreas. It is an endogenous receptor for lysophosphatidylcholine (LPC), which is is a lipid mediator involved in insulin secretion from pancreatic ? cells. GPR119 may participate in this insulin secretion, suggesting that it may be potential target for new anti-diabetic drugs."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
This antibody recognizes ~37 kDa of GPR 119 protein. It reacts with mouse and rat. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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