Antigen Preparation
A synthetic peptide corresponding to the internal sequence (a portion from 120aa-180aa) of mouse NEFA protein. This sequence is identical to mouse and rat.
Background
"NEFA, a 420 amino acid protein (also known as NUCB2, nucleobindin 2, p54), is a DNA-binding protein localizing in the Golgi Apparatus. This protein has been implicated in various functions, including Ca2+ homeostasis, hypothalamic regulation of feeding and TNF-receptor-1 (TNFR1) shedding. NUCB2 has been shown to interact with both necdin and ARTS-1, both through the EF-hand motif. The NUCB2-ARTS-1 complex is required for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been identified as a novel satiety molecule in the hypothalamous associated with melanocortin signaling in the central nervous system.NUCB2 and its N-terminal 82-amino acid cleavage fragment, termed nesfatin-1, have been shown to induce suppression of food-intake in rats."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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