Antigen Preparation
"A synthetic peptide corresponding to the internal sequence (a portion from 100aa-190aa) of human Legumain protein. This sequence is identical to mouse, human and rat."
Background
"Legumain, a 433 amino acid protein with 17 aa signal sequence, is a cystein protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic . Protein expression occurs after monocytes differentiate into dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This novel asparaginyl endopeptidase has been observed to be highly expressed in several types of tumors including colorectal cancer. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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