Antigen Preparation
"A synthetic peptide derived from the adjacent to C-terminus of JAK2 protein. This sequence is identical in JAK2 from human, rat and mouse origins."
Background
"The Janus tyrosine kinase family is consists of four members, JAK1, JAK2, JAK3 and Tyk2. Upon the cytokine ligand binding to its the receptors, the dimerization of the receptors occurs followed by phosphorylation of the receptors. The phosphorylation of receptors recruites the tyrosine kinase physically associated to the dimerized receptors, and the phosphorylate the kinase, thus activates the signal transducer and activator of thranscription (STAT1, 3, 5) and the other signal proteins."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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