Raf-1
v-raf-1 murine leukemia viral oncogene homolog 1; c-Raf; RAF proto-oncogne serine/threonin-protein kinase
Download Product PDF
MSDS Download Product PDF
Antigen Preparation
A synthetic peptide derived from internal segment of human Raf-1
Background
"Raf-1 is the cellular homolog of viral raf gene (v-raf). It is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
|
SPECIFICITY
"This antbody recognizes Raf-1 protein. It reacts to human, mice and rat. The other species not tested."
|
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
|
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
|
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|