Antigen Preparation
"A synthetic peptide surrounding to the C-terminus of p19 protein from human. This sequence is identical to human, mouse and rat origins."
Background
"The p19 INK4d, a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, plays a dual role in the DNA damage response, inhibiting cell proliferation and promoting DNA repair. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. The p19INK4d has been reported to become upregulated in response to UV irradiation and a great variety of genotoxic agents."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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