Antigen Preparation
A full length of GST recombinant protein.
Background
"Many oligo peptides or tag proteins are widely used as a tag to monitor the protein expression, such as FLAG, c-Myc, E-tag, His-tag, GFP, GST, and etc. These tag antibodies provide tools to localize gene products in a variety of cell types to study the topology of proteins, and protein complexes, and to identify associated proteins. In addition, this antibody allows characterizing of newly identified, lowing abundance or poorly immunogenic proteins when protein specific antibodies are not available. This GST antibody can be used for pGEX expression vector for the synthesis of fusion proteins between GST and targeted protein expression in both plaques and western blots as well as for immunoaffinity purification of GST fusion proteins expressed in E. coli."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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