CREB
cAMP responsive element binding protein
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Antigen Preparation
A synthetic peptide corresponding to the epitope of RPSYR with a single phosphorylation site Ser133.
Background
"CREB is a bZIP transcription factor that binds the cAMP response element (CRE) and activates target genes. It is involved in many extracellular signals resulting in regulation of a variety of cellular responses. Phosphorylation of CREB at Ser133 by various kinases such as PKA, PKC, CaM kinase II and IV is necessary for CREB signaling."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
This antibody only recognizes ~40 kDa of human CREB protein. It also reacts with rat CREB protein. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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