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p16INK4a (CT)

Cyclin-dependent kinase inhibitor 2A; CDKN2A; P14;

Antigen Preparation

A synthetic peptide corresponding to the C-terminus of human p16INK4a protein.

Background

The INK4 (inhibitor of cyclin-dependent kinase 4) family consists of four tumor-suppressor proteins: p15(INK4B), p16(INK4A), p18(INK4C), and p19(INK4D). While their sequences and structures are highly homologous, they show appreciable differences in conformational flexibility, stability, and aggregation tendency. p16 (INK4a, CDKN2A, cyclin dependent kinase inhibitor 2A) is a tumor suppressor gene that inhibits cdk4 and cdk6 which phosphorylate the Rb protein. P16INK4a overexpression has been demonstrated in cervical cancers as a result of functional inactivation of pRb Thus, this antibody can be an important marker for dysplastic squamous and glandular cells of the cervix.

PURIFICATION The Rabbit IgG is purified by Epitope Affinity Purification	SPECIFICITY This antibody recognizes ~16-18 kDa of human p16INK4a protein. The other species are not tested
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.	STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

1029

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

5-10 µg/ml

Order Info

Catalog #:	500-6314R
Lot #:	See the label
Size:	100ug
Host:	Rabbit
Clone:
Isotyping:	Rabbit IgG
Applications:	WB, IP, IHC-P
Reactivity:	Hu,
Price:	$ 279.00

REFERENCES

N Murphy, et al. p16iNK4A as a marker for cervical dyskaryosis: CIN and cGIN in cervical biopsies and ThinPreTM smears. J. Clin. Pathol. 2003; 56-63.