Keratin 18
CK-18; cytokeratin 18; KRT18
Antigen Preparation
Human breast cancer PMC 42 cells
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
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SPECIFICITY
Keratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with keratin 8. It was reported that tissues from gastrointestinal tract are positive for both keratin 8 and 18 but do not contain keratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for keratin 18.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
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STORAGE
The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
5-10 µg/ml
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