Antigen Preparation
A synthetic peptide from C-terminus of human c-Met protein
Background
"The c-Met oncogene, a member of the tyrosine kinase superfamily, was originally isolated from chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a trans-membrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The mature c-Met protein is a disulfide-linked heterodimer with Mw 190 kDa composed of a heavily glycosylated domain, a single transmembrane domain and a cytoplasmic tyrosine kinase domain. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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