Antigen Preparation
A synthetic peptide corrsponding to the internal segment of human p63
Background
"P63 is a transcription factor p63 that is widely expressed nuclear protein. Alternate promoters encode two main variants, TAp63 and ΔNp63, which are further spliced into at least five isoforms, designated α, β, γ, δ and ε, due to alternative splicing events at the carboxy-terminus. TAp63 is transcribed from an upstream promoter containing a similar transactivation domain to p53, while ΔNp63 is transcribed from a promoter located on intron 3, that results in a unique transactivation domain and distinct biological functions. Considered to be oncogenic, ΔNp63 is required for cell growth and survival and can be dominant-negative over TAp63 and p53. TAp63 can transactivate some p53 target genes and is primarily responsible for tubulogenesis and cyst formation."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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