Antigen Preparation
A synthetic peptide corrsponding to the internal segment of human retinoblastoma RB
Background
"RB, retinoblastoma, is a negative regulator of the cell cycle and was the first tumor suppressor gene found. It is a key regulator of the cell cycle during the transition from the G1 to S phases. RB also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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